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  CAPITOLINA ROOM
13.00-13.30 REGISTRATION

13.30-14.30 OPENING CEREMONY

  Conference Introduction & Opening
C. U. Casciani
R. Lauro
V. Cervelli

  Welcome Remarks
M. Baldi – Lazio Region Council Member
G. Novelli – Rector of University of Rome “Tor Vergata”
N. Pallua – EASAPS, ESPRAS Executive Committee Member
F. Moschella – President of the Italian Council of Professors in Plastic Surgery
R. Lala - President of Rome’s Medical Association
O. Schillaci – President of Faculty of Medicine, University of Rome “Tor Vergata”
L. Palombi – Director of Biomedicine & Prevention Department, University of Rome “Tor Vergata”
14.30 - 16.15 BIOLOGICAL INDIVIDUALITY, EPIGENETICS OR PERSONALIZED MEDICINE: FROM OBESITY TO FAT TRANSPLANT
  Chairmen: G. Novelli (Rome, Italy), A. Orlandi (Rome, Italy)

  Tissue/organ-specific differentiation of human induced pluripotent stem (iPS) cells
D.C. Gruenert (San Francisco, USA)
  Nuclease mediate genome editing for therapeutic purposes
M. Porteus (Stanford, USA)
  Development of human lung scaffolds to test the regenerative properties of adult stem cells
J.W. Lee (San Francisco, USA)
  The stromal vascular fraction mediates improvement in disease severity in the experimental Autoimmune Encephalomyelitis (EAE) model of multiple sclerosis
B. Bunnell (New Orleans, USA)
  Adipose derived stem cells: understanding subpopulations
P. Rubin (Pittsburgh, USA)
  Discussion


16.15 -16.30 COFFEE BREAK


16.30 - 19.00 TRANSLATIONAL MEDICINE & SURGERY
  Chairmen: V. Cervelli (Rome, Italy), F. Moschella (Palermo, Italy)

  Understanding and interpreting scientific studies on adipose stem cells
P. Rubin (Pittsburgh, USA)
  Adipose-derived cells: standardization and application
B. Bunnell (New Orleans, USA)
  Platelet-rich plasma potentiates insulin-induced Akt-dependent adipogenic differentiation of adult adipose tissue-derived stem cells
A. Orlandi (Rome, Italy)


Human adipose sphere-derived stem cells increase the mesenchymal potential: terapeutical implication
G. Stassi (Palermo, Italy)
  Clinical and experimental experience with lipogems treatment
C. Tremolada (Milan, Italy)
  The Lipogems system application in orthognathic surgery patients
M. Raffaini
  Autologous fat transfer and growth factors: risk or benefit
N. Pallua (Aachen, Germany)
  Immunotargeting of cancer initiating cells in pancreatic ductal adenocarcinoma
C. Ferrone (Boston, USA)
  CSPG4-mediated targeting of cancer initiating cells in triple negative breast cancer
S. Ferrone (Boston, USA)
  Discussion


CAPITOLINA ROOM
8.45 - 10.00 REGENERATIVE SURGERY IN WOUND CARE: BIOLOGICAL IMPLICATIONS
  Chairmen: F. Bassetto (Padua, Italy), L. Teot (Montpellier, France)
  Growth factors in wound healing: an unmet need
L. Teot (Montpellier, France)
  Gene therapy in wound healing: where are we so far?
G. Giatsidis (Padua, Italy)
  State of the science and art of regeneration
D. Orgill (Boston, USA)
  Integration kinetix of collagen-GAG dermal regenerative scaffolds: effects of varying thickness and sub-atmosferic tressure dressings
L. Lancerotto (Padua, Italy)
  The use of epidermal growth factor in diabetic foot ulcers
S. Baghaki (Istanbul, Turkey)
  Evolution of mechanical forces in wound healing:prevention of complications in surgical wounds in the obese patient
F. Bassetto (Padua, Italy)
  Discussion

10.00 - 11.25 REGENERATIVE SURGERY IN WOUND CARE: FROM BIOLOGICAL TO
CLINICAL APPLICATIONS
  Chairmen: C. Alfano (Perugia, Italy), L. Vaienti (Milan, Italy)
  Use of fat grafting as an adjuvant therapy in burn wounds
N. Piccolo (Goiania, Brazil)
  Expanding regenerative surgery in wound healing
S. Baghaki (Istanbul, Turkey)
  A new alternative A-PBMNC methodology in CLI treatment
B. De Angelis (Rome, Italy)
  Fibroplasts: how, when and where to apply
T. Voukidis (Athens, Greece)
  Burn scar lipofilling: immunohistochemical and clinical outcomes
P. Palombo (Rome, Italy)
  Application of enhanced stromal vascular fraction and fat grafting mixed with PRP in lower
extremity ulcers

V. Cervelli (Rome, Italy)
  From micro lobules to large lobules fat grafting
A. Mojallal (Lyon, France)
  Discussion


11.25 - 11.45 COFFEE BREAK


11.45 - 12.00 LECTURE
  Risks & complications in regenerative plastic surgery
N. Scuderi (Rome, Italy)

12.00 - 13.15 HOW TO AVOID COMPLICATIONS IN REGENERATIVE SURGERY?
  Chairmen: S. Bruschi (Turin, Italy), M. Pascone (Bari, Italy)
  The correction of facelift and blepharoplasty complications with fat grafting
S. Coleman (New York, USA)
  Ultrasound assisted lipotransfer over implant
A. Mojallal (Lyon, France)
  Can fat reabsorption be considered a compilation?
R. Mazzola (Milan, Italy)
  Lidocaine and adrenaline effect on human adipose stem cells
R. Roche (Marseille, France)
  Complications & risk management in the treatment of diabetic foot
L. Uccioli (Rome, Italy)
  Sonographic and radiological quantification of breast oil cysts, fat necrosis and calcifications associated to cell-enhanced fat grafting
R. Llull (Palma de Mallorca, Spain)
  Discussion

13.15 - 14.00 LUNCH
14.00 - 15.25 CLINICAL APPLICATIONS OF FAT GRAFT
  Chairmen: F. D’Andrea (Naples, Italy), N. Piccolo (Goiania, Brazil)
  Harvesting of fat graft at different pressures: does the asspiration pressure modify the viability rate of fresh or cryopreserved adipocytes and ADSC?
F. Moscatiello (Barcelona, Spain)
  Autologous fat micro graft in management of abnormal scars
S. Moshref (Jeddah, Saudi Arabia)
  From lipofilling to microfilling: how to improve fat grafting?
R. Roche (Marseille, France)
  Physiology of fat tissue and how increase the result of the autologous fat transplantation
G.H. Leibaschoff (Dallas, USA)
  Bringing adipose-derived products from the cell factory to the patient
G. Soldati (Lugano, Switzerland)
  Personal experience in the use of fat nanografting in facial rejuvenation
E. Bartoletti (Rome, Italy)
  Third generation UAL as alternative fat harvesting technique - its impact on adipocyte viability and SVF quality
K. Schlaudraff (Geneve, Switzerland)

  Discussion

15.25 - 17.10 REGENERATIVE SURGERY IN CRITICAL AREAS
  Chairmen: A. Faga (Pavia, Italy), N. Pallua (Aachen, Germany)

  Regenerative influence of fat grafts on nerves
S. Coleman (New York, USA)
  Fat tranfer in reconstructive surgery- multipotential replacement tool
E. Siolo (Ballito, South Africa)
  Autologous fat transplantation from non fatty areas in supraponderal patients
T. Mugea (Cluj-Napoca, Romania)
  New options in regenerative surgery of the ano genital area for Lichen Sclerosus disease: 7 years experience
F. Casabona (Genoa, Italy)
  Treatment of congenital muscular torticollis with intramuscular fat grafting
J. Monreal (Madrid, Spain)
  Long-term follow-up in rigenerative treatment for vulvar dystrophies
M.G. Onesti (Rome, Italy)
  Facial soft tissue restoration and contouring by enriched A-PBMNC fat grafting
D. Bottini
  Use of fat grafting as an adjuvant therapy in complex wounds and critical areas
N. Piccolo (Goiania, Brazil)
  Avoiding complications in large volume fat transfer
D. Del Vecchio (Boston, USA)
  Discussion

17.10 - 17.25 LECTURE
  Legislative aspects in the use of SVF in regenerative surgery
S. Fiorentino (Rome, Italy)
17.25 - 19.00 FIRST CONSENSUS CONFERENCE: FAT GRAFT STANDARDIZATION
  Chairmen: V. Cervelli (Rome, Italy), S. Coleman (New York, USA), R. Llull (Palma de Majorca, Spain)
AVENTINA ROOM
10.00 – 11.30 WORKSHOP

14.15 - 15.35 CLINICAL USE OF FAT GRAFT IN REGENERATIVE SURGERY
  Chairmen: A. Cordova (Palermo, Italy), G. Petrella (Rome, Italy)
  Fat implants for the face for patients with or without scars
R. Garofalo (Rome, Italy)
  Immediate reconstruction with fat grafting in breast conserving surgery - Avoid breast sequelae
V. Gonzalez (Valencia, Spain)
  Lipofilling in breast reconstruction: the innovative BEAULI technique
A. Mori (Rome, Italy)


Lipofilling versus free flaps in facial atrophy
M. Pagnoni (Rome, Italy)
  Fat and epidermal cell suspension grafting: a single-stage technique for skin graft sequelae correction
E. Migliano (Rome, Italy)
  Magnetic resonance imaging and ultrasound evaluation after breast autologous fat grafting combined with platelet-rich plasma
V. Liberto (Rome, Italy)
  Revision of 175 augmentation faloplasty patients
J. Monreal (Madrid, Spain)
15.35 - 16.45 THE CLINICAL ROLE OF GROWTH FACTORS
  Chairman: G. Adorno (Rome, Italy), R. Serafini (Rome, Italy)
  Platelet rich plasma. Comparative differences in methods and preparation tubes the right choice
M. Hadjipieras (Papagou, Greece)
  Regenerative surgery: treatment of painful neuroma
C. Di Pasquali (Rome, Italy)
  Dermal regeneration templates: an alternative choice for wound treatment
I. Bocchini (Rome, Italy)


Adipose derived stem cells and PRP in enhancing the maturation of post burn and post traumatic scars: a clinical and histopathological study
H. S. Abulhassan (Alexandria, Egypt)
  Autologous fat transfer and platelet-rich plasma in the treatment of the genital lichen sclera-atrophic. Preliminary results from a multidisciplinary protocol
M. Tedesco (Rome, Italy)
  Aesthetic application of PRL: soft tissue defects and scars treatment
G. Sanese (Rome, Italy)
16.45 - 17.35 TECHNICAL PEARLS IN REGENERATIVE SURGERY
  Chairman: P. Persichetti (Rome, Italy), M. Valeriani (Rome, Italy)
  The effect of the harvesting cannula diameter on the quality of lipoaspirate
M. Salgarello (Rome, Italy)
  Comparison yield of SVF cells isolated after aspiration with different cannulas and at different
levels of depth

A. Trivisonno (Rome, Italy)
  How can we help the skin by cosmetic devices?
F. Tomaselli (Rome, Italy)


Adipose tissue as a novel resource for bone regeneration: analysis of osteogenic potential of adipose-derived mesenchymal cells
S. Pagani Bologna, Italy)
17.35 - 18.45 CRITICAL AREAS AND LOWER EXTREMITY ULCERS
  Chairmen: G. Caselli (Rome, Italy), A. Ippoliti (Rome, Italy)
  Usefulness of autologous fat grafting in lower extremely reconstruction
J. Monreal (Madrid, Spain)
  Combined regenerative approach (PDGF + autologous cell suspension) for definitive repair of vasculitic ulcers
S. Polotto (Genoa, Italy)
  Regenerative of critical areas using a cellular dermal matrix
A. Beltrami (Buenos Aires, Argentina)


Our experience in the use of mesenchymal stem cells isolated from lipoaspirate for the treatment of chronic skin ulcers
E. Raposio (Rome, Italy)


Core fat graft injection in depressed scar augmentation
M. Abdelrahman (Ismailia, Egypt)


The role of adipose derived stem cells in enhancement of peripheral nerve injuries repair
H.M. Ayad (Tanta, Egypt)


Discussion
CAPITOLINA ROOM
9.00 - 10.25 FACE TO FACE: FAT GRAFTING FROM RECONSTRUCTION TO AESTHETICS
  Chairmen: S. Coleman (New York, USA), G. Iannetti (Rome, Italy)

  Craniofacial reconstruction with fat grafting
P. Rubin (Pittsburgh, USA)
  Periocular fat grafting: the PDF technique and its magic properties
M. Pelle Ceravolo (Rome, Italy)
  The use of nanografts in aesthetic surgery
J. Planas (Barcelona, Spain)
  Versatility of fat graft in facial area: new experience
M. Klinger (Milan, Italy)
  Adipose stem cells and lipografting: evidence based medicine
A. Sterodimas (Athens, Greece)
  Application of platelet rich lipotransfer and insulin in soft tissue defects of the face
V. Cervelli (Rome, Italy)
  Micro fat injection: from systemic sclerosis to facial rejuvenation
G. Magalon (Marseille, France)
  Discussion


10.25 - 10.45 COFFEE BREAK


10.45 - 12.30 THE BREAST NATURAL FILLER: FAT GRAFTING
  Chairmen: Z. Arnez (Trieste, Italy), A. Portincasa (Foggia, Italy)
  Eighteen years of shaping breasts with structural fat
S. Coleman (New York, USA)
  Fat grafting in breast reconstruction, 7 years experience
R. De Vita (Rome, Italy)
  Enriched fat grafting in breast augmentation: long lasting results
D. De Fazio (Milan, Italy)
  Increasing percent volume maintenance in large volume fat transplantation-the role of cell protectants
D. Del Vecchio (Boston, USA)
  Combined mastopexy and augmentation with autologous fat grafting: first results with lipopexy
A. Grisotti (Milan, Italy)
  The fat capsule: avoiding radiotherapy-induced damage in immediate prosthetic breast reconstruction
D. Ribuffo (Cagliari, Italy)


The combined use of enhanced stromal vascular fraction and platelet-rich plasma improves fat grafting maintenance in breast reconstruction: clinical, in vitro and instrumental evaluation
P. Gentile (Rome, Italy)


New perspectives in plastic surgery: adipose derived stem cells and enriched fat grafting
in breast surgery

M. Zocchi (Turin, Italy)
  Regenerating a breast mound with external expansion and fat grafting
K. Khouri (Miami, USA)
  Discussion


12.30 - 13.20 RESHAPING THE BUTTOCKS: CONCAVE & CONVEX
  Chairmen: C. D’Aniello (Siena, Italy), F. Stagno D’Alcontres (Messina, Italy)
  Gluteal augmentation assisted by stromal enriched lipograft
A. Sterodimas (Athens, Greece)
  Body and buttocks reshaping with lipofilling
D. De Fazio (Milan, Italy)
  Curves in buttocks contouring
C. Bernardi (Rome, Italy)
  Thighs and buttock contouring: 25 years experience in liposculpture and fat grafting
M. Gasparotti (Rome, Italy)
  Discussion

13.20 - 14.15 LUNCH
14.15 - 16.15 EASAPS SYMPOSIUM SURGICAL PEARLS: SPECIAL TECHNIQUES
IN LIPOTRANSFER
  Chairmen: V. Cervelli (Rome, Italy), N. Pallua (Aachen, Germany)
  Concentrating fat grafts for facial structural fat grafting
S. Coleman (New York, USA)
  Hand rejuvenation
G. Magalon (Marseille, France)
  Trends in lipostructuring of fibrotic diseases
F. Bassetto (Padua, Italy)
  Lipostructuring versus micrografting
N. Pallua (Aachen, Germany)
  The use of platelet rich lipotransfert (PRL) and enhanced stromal vascular fraction (e-SVF) in aesthetic plastic surgery
V. Cervelli (Rome, Italy)
  The lipografter: a practical system for efficient atraumatic fat harvesting, processing and micro-grafting
K. Khouri (Miami, USA)
  Discussion


16.15 - 17.30 BREAST & BODY RECONSTRUCTION
  Chairmen: G. De Santis (Modena, Italy), C. Rubino (Salerno, Italy)

  The reverse abdominoplasty and fat transfer (RAFT)/ procedure: a game changer in breast surgery
K. Khouri (Miami, USA)
  Breast reconstruction/augmentation by reverse expansion and fat graft - a Romanian experience
(the BRAVA-system)

D. Jianu (Bucharest, Romania)
  Evolution of fat grafting to the breast: basic science and surgical technique
G. Rigotti (Mantova, Italy)
  Water-jet assisted fat grafting to the female breast: preliminary experiences
M. Stabile (Cremona, Italy)
  The thoracodorsal system as carrier for expandable vascularized autologous matrix
F. Santanelli (Rome, Italy)
  Local recontouring after autologous and implant based BR with fat grafting or resorbable mesh: a feasible alternative?
S. Pompei (Rome, Italy)
  Discussion


17.30 – 17.45 CLOSING REMARKS
  S. Coleman
V. Cervelli